A Validated RP-HPLC-UV Method for Simultaneous Estimation of Ceftriaxone and Sulbactum in Rat Plasma

A reverse phase-liquid chromatographic method with UV detection is developed for simultaneous estimation of ceftriaxone sodium and sulbactam sodium in rat plasma. A simple protein precipitation technique was employed for the extraction of drugs from blank plasma. Chromatographic separation of the two drugs was achieved on C18 column (250 mm X 4.6 mm, i.d, 5 μm) using a mobile phase consisting of 10mM potassium dihydrogen orthophosphate buffer (pH- 5) and acetonitrile (90:10% v/v). The developed liquid chromatographic method eluted both the drugs with a reasonable retention time, acceptable symmetric peak shape and good resolution. The developed bio-analytical method was linear, accurate, and precise over the concentration range of 20-150 μg mL-1 for ceftriaxone and 10-75 μg mL-1 for sulbactam. The developed method was successfully applied to monitor ceftriaxone and sulbactam sodium concentrations in rat plasma.